Fusarium inoculation into the tissues of the tree is actually the bacteria that cause disease. Therefore it against a tree aloes produce resin called fitoaleksin so do not spread germs to other tree tissues. Over time, the resin was hardened at the point of xylem and phloem vessels corner - tree organ which distributes food browned and fragrant when burned.
the type isolate the disease-forming aloes will vary according to
climatic and environmental conditions, the inoculant providers need to
do isolation type of diseases that produce gaharu prospected. Isolation was carried out on natural aloe plant that was in the forest area surrounding the development. To
that end, should begin with the field observations to study aspects of
aloes that grow naturally and to isolate and identify the type of
disease from the infected tree.In order to successfully develop the inoculant-forming aloes, specific techniques are required. For
this, the indispensable role of local government agencies or
institutions, universities, private entrepreneurs and investors or local
area as the perpetrators of inoculant production. The stages of development techniques inoculant as follows:
• Choose natural aloe which had been infected with the disease-forming microbes aloes.• Take a piece of branch or peeling aloe tree selected. Peeling pieces of branches or stems is called "preparations".•
Take it to the laboratory preparation and effort so that its
temperature and humidity is maintained by means incorporated in the ice
box.• Develop and spores from preparations branch or trunk is in the media in order to identify microbial species as a pure culture.•
Develop spores and mycelium of pure cultures into the dense media such
as sawdust and tree aloes or in liquid medium containing ang has macro
and micro elements as the energy of life.•
Enter the spores into the media culture incubator and incubator
temperature and humidity conditioning of breeding is in optimal
condition, the temperature of 24 - 32c and 80% humidity. Allow approximately 1-2 months.• Place the spores are sdah bred into a container glass bottles, plastic bottles, or bottles of infusion used.• Store the bottle in the freezer incubator. This inoculant is ready inoculated into the aloe plant. Mechanical inoculation with inoculant of aloe will vary according to the form inokulannya. In the implementation of the aloe penginokulasian this, be aware of age and trunk diameter. Minimum limit on the inoculation of a tree can be characterized by flowering trees. Usually the age of the plant is about 4-5 years or have reached a trunk diameter of 8-10 cm. Reviewed following inoculation technique using solid and liquid inoculants.
Inoculation with solid inoculants.Aloe inoculation technique using solid inoculant carried in the following way:
• Make a hole in the stem wood of aloes to use a drill. Borehole diameter of about 0.8 to 10 mm. Optimal depth of drilling is to be adjusted with stem diameter, typically about 5 cm. Each rod made a lot of holes spaced drill holes about 20 cm.• Clean hands of perpetrators of inoculation with water until clean and rinsed with alcohol before the inoculation.• Enter a dense inoculation into each hole. Adjusted by the amount of inoculant hole depth. As a rule, income is made up holes filled with the inoculant. In order for revenues to be easy, use a piece of wood or bamboo whose size matches the size of the hole diameter.• Cover any holes that have been given mnghindari inoculant for the entry of water into the hole. Hole closure was done with wooden pegs aloes. Closure can be done with "lily-night"
Inoculated with liquid inoculant.Inoculation technique using liquid inoculants carried in the following way:• Perform the drilling at the base of the tree trunk with his side down. Drilling depth adapted to the tree trunk diameter, usually 1/3 the diameter of the rod. While the drill bit is used together with the IV measuring approximately 0.5 cm. Infusion hose is usually supplied producers inoculant inoculant at time of purchase. However, if not available, the infusion tube can be provided by the farmers themselves.• Enter the IV that is on the bottle of liquid inoculant into the hole.• Adjust the amount of flow of the liquid inoculant. Stop the flow of fluid infusion when the inoculant was out of the hole.• Close the hose around the edges of the infusion by using the "candle night".•
Repeat setting the entry flow of fluid infusion into the hole every 1-2
days, depending on the state of the fluid in the hole. Flow arrangement made when a hole is no longer fluid inoculation.• Implement this penginokulasian liquid inoculant in the bottle until the infusion is finished. Penginokulasian repeated inoculation with a new bottle, if there are no signs of physical and physiological death.